DNA extraction and amplification in 1 hour Optimize proprietary lysis buffer Single tube - no organic solvents or clean up procedures Enhanced Accuris Hot Start Polymerase included The Accuris Mammalian Genotyping Kit is quick and easy to use - add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR Accuris Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed - just add sample and primers.