Conventional milking machines for mice and rats are few in number and difficult to operate, so most of them are used to collect milk from the stomach of lactated baby.This one-hand milking device for mice and rats makes milking much easier and without using baby animals. The device covers the nipple area of the animal to be milked with a tube with a trumpet-shaped tip. A separate suction cube is used to inhale the air from the milking tube. Negative pressure inside of the tube sucking air, will collect the milk directly from mother’s nipple.